Find a mission statement for a hospital near you and share it here.
Discuss how it demands that the organization focus on quality.
How would you implement the mission statement as part of the strategic plan?
Restriction Enzymes persuasive essay help English assignment help
The restriction enzyme, also known as a restriction endonuclease, is a protein produced by bacteria that slices DNA at a particular site. In other words, it identifies one or some target sequences and cuts DNA at nearly those arrangements. Notably, most restriction enzymes make staggered cuts and produce ends that have single-stranded DNA projections. Nevertheless, some of them create blunt ends. Scientists’ first breakthrough on the road to genetic engineering was fruitful after Herbert Boyer’s efforts on the restriction endonucleases at the University of California. Importantly, these enzymes occur in large numbers of different bacterial classes. Roberts et al. (2014) asserts that they serve as part of the standard defense mechanism that prevents bacterial cells from being invaded by external DNA molecules like those found in viruses. For instance, when a virus attacks a single-celled bacterium, these enzymes are released such that they cut the intruding DNA into little, non-threatening pieces. Most importantly, this nuclease has the aptitude to discriminate between its DNA and the intrusive DNA; else the cell would terminate its own DNA.
The recognition process takes place due to two elements. Firstly, there is two particular nucleotide arrangement (As and Ts, Cs, and Gs). Notably, these sequences act as nuclease targets called restriction sites. Secondly, a shielding chemical signal is present that is placed by the cell on all the aimed course that occurs in its DNA. The signal alters the DNA and averts the nuclease from cutting. As such, the nuclease may chop the invading DNAs that lack protective signal. The restriction enzymes have the extraordinary aptitude to distinguish particular DNA arrangements base pairs-As and Ts, Gs, and Cs. Moreover, they can act as a molecular blade, such that they severe the DNA at just where they detect the sequence of hereditary letters. Restriction enzymes are prevailing because they exist in large numbers and each of them acts in a unique As, Ts, Cs, and Gs sequence.
Area of Use
Restriction enzymes aptitude to reproducibly cut DNA at precise sequences has become a widespread tool used in molecular genetic methods. For instance, these enzymes may be useful to map DNA Genome or fragments. Mapping is the process of determining the genome’s restriction enzyme sites’ order. Notably, the maps create a foundation for further genetic examination. The enzymes are also regularly used in the verification of the distinctiveness of a precise DNA fragment, which is based on the identified restriction enzyme site it encompasses (Nisbet, 2004). One of the essential uses of restriction enzymes is the production of recombinant DNA molecules. These are DNAs that have genes or fragments from two dissimilar organisms. Precisely, the bacterial DNA, which is in the form of a placid, combines with another piece of DNA from a different organism. Restriction enzymes assist at various points in this process.
Restriction enzymes aid in the incorporation of DNA from the trial organism, to prepare the DNA for cloning. Consequently, a bacterial virus or plasmid is digested with an enzyme that produces well-matched ends. These ends may or may not have an overhanging sequence. The DNA from the investigational organism is joined with the plasmid of virus DNA through the use of an enzyme called DNA ligase. As above-mentioned, restriction enzyme digestion verifies the recombinant DNA molecule. Also, these enzymes are applied in the procedures of categorizing individuals or strains of specific species (Shapiro, 1997). For instance, the process of splitting large DNA fragments is referred to as gel electrolysis. Typically, these fragments come from the digestion of a microbial genome that has a rare-cutting restriction enzyme. As such, the process produces reproducible arrangements of DNA bands that aid in distinguishing various bacteria strains. Moreover, they help determines if a widespread disease outbreak emanated from a specific strain.
Cloning human beings using the processes mentioned above has received diverse reactions based on ethical considerations. For instance, the cloning of “Dolly,” a sheep cloned from an old sheep’s mammary gland cell nucleus in 1997 was successful; hence, talks about the probability of human cloning being developed. While there have been minimal cases of attempted human cloning, animal cloning has received strong opposition. Bioethical principles demand that before conducting long-standing and methodical research, all possible effects on nature should require proper consideration. Therefore, using animals and plants for cloning research in most cases translate to irresponsible handling of the plants and animals world, not to mention the ecosystem. Thus, in 1986 the Organization of Economic Cooperation and Development (OECD) formulated the Recombinant DNA Safety Considerations and also a couple of recommendations. Hence, these considerations and recommendation guide researchers on ethics associated with this technology.
When John Gurdon, a developmental biologist at Oxford University cloned frogs in 1960, he did it limitedly. Gurdon demonstrated that a nucleus from the intestinal lining cell of a tadpole is transferable to an enucleated fertilized egg. He found out that an adult cell’s nucleus could support progress up to the tadpole stage. However, he could not coax a nucleus from the cell of an adult amphibian. In the 1980s, researchers attempted to commercialize livestock cloning from nuclei extracted from fetuses and embryos. However, their efforts did not succeed because the cloned animals were unhealthy newborns that did not endure for long. Currently, cloning is partial to research and cloning humans would yield similar results. Moreover, using human and animal for research without the guarantee of positive results is ethically unwanted. Since survivability is as minimal as in animal cloning, it is unrecommended.
- Nisbet, M. C. (2004). Public opinion about stem cell research and human cloning. Public Opinion Quarterly, 68(1), 131-154. Retrieved from https://academic.oup.com/poq/article/68/1/131/1855075
- Roberts, R. J., Vincze, T., Posfai, J., & Macelis, D. (2014). REBASE—a database for DNA restriction and modification: enzymes, genes and genomes. Nucleic acids research, 43(D1), D298-D299. Retrieved from https://academic.oup.com/nar/article/43/D1/D298/2436339
- Shapiro, H. T. (1997). Ethical and policy issues of human cloning. Science, 277(5323), 195-196. Retrieved from http://science.sciencemag.org/content/277/5323/195.full
250 Words Discussion Board
Erving Goffman Is One Of The Most Important Sociological Thinkers In Modern Sociology. His Biggest Contribution To Sociology Was The Dramaturgical Lens Of Viewing Social Interaction, A Theory That He Developed By Just Watching People In Their Everyday In
Erving Goffman is one of the most important sociological thinkers in modern sociology. His biggest contribution to sociology was the dramaturgical lens of viewing social interaction, a theory that he developed by just watching people in their everyday interactions. In the pieces that we read for this class, we talked about these elements of dramaturgy and how they play out in real life. For this essay question: (1) define the concepts of impression management and audience segregation and then explain how and why people use impression management and audience segregation in daily social life (2) give a detailed example of how you used impression management and audience segregation in the last week and finally (3) explain how technology has changed features of impression management and audience segregation. Give one positive and one negative example. Be as complete and thorough as possible with your answer.
150 words agree or disagree So, when it comes to the difference between male and female terrorism there is a difference and, the woman’s role is to help and support their husbands, raise their children. Women have also gone as far as marrying someone as an alliance stand point to unit different tribe that have the same beliefs and values. These are like big picture out looks as to what role women hold in a terrorist organization. ?On a smaller scale, women have taken on operational roles in the planning and executions of attacks, including a suicide bombers. As the principal focus in understanding (global) jihadism is often on perpetrators and leaders – positions in which women are underrepresented in Jihadist movements – women’s facilitative and supportive contributions are often poorly understood and assessed.de Leede, S. (Sep 2018). There has been reports that have showed that between 145-300 women from all over Europe who have left their countries to go join up with the self-style Islamic State, (IS) the roles that these women play in the (IS) is wives and mothers there are some cases where these women have played roles as educators or administrative people all the way up to medical support. So, women do play a different role but they can also play somewhat of the same role like with suicide bombers. Mostly the women play the role of mother and wife to the men and children teaching them the ways of that life style. Speaking freely, I don’t think that the US counterterrorism unit should differ between gender to me it doesn’t matter what race, color, sex, religion you are if you’re out there trying to hurt people because you don’t like them or they don’t agree with your religion beliefs and you think they need to die for that because they aren’t pure of heart or whatever you want to call it. That doesn’t give you the right to strap a bomb vest on yourself and go blow up a whole crowed of people and whether you male or female if you are caught in these types of acts I think you should get the same type of punishment across the board. To me it should be a set standard of punishment if your caught and you are guilty then yeah you pay the price. I mean did you give all those innocent people the same respect so why should we. I try to look at it as a big picture. Congrats everyone on this class we made it. Jordan de Leede, S. (Sep 2018). Women in Jihad: A Historical Perspective. Retrieved from https://icct.nl/wp-content/uploads/2018/09/ICCT-deLeede-Women-in-Jihad-Sept2018.pdf
3-Page Interpretation Paper
Who can do a 3-page Interpretation paper concerning social, political, and cultural information about the early 19th century? The paper is on “The Oral Tradition.” In your own words, explain ways in which the poems, rhymes, songs, and narratives contribute to the body of African-American literature? Identify features of orality. Conclude with the most surprising or disturbing fact you have learned. In other words, what is your takeaway from this unit. Use in-text citations and provide a Works Cited page for examples and texts used. Cite sources if critical quotations are used to back up your claims.
For “Prof. Nicholas” – CJA/444 – Criminal Justice Organizational Trends Paper
Write a 700- to 1,050-word paper on criminal justice organizational trends. Include the following information: Compare the use of privatization, e-corporation, and militarization of criminal justice organizations with current organizational structures. Compare historical and traditional organizational behavior theories, and the effect of societal and organizational diversity in criminal justice organizations. Using the Criminal Justice Motivational Theories Matrix, explain how one or more theories of motivation affect criminal justice organizations. (You did the Matrix 2 weeks ago, I will attach a copy of it so you can use it for review). Include at least three peer-reviewed articles from the University Library. (University is the University of Phoenix) Format your paper consistent with APA guidelines. Two references required. AS ALWAYS, ABSOLUTELY NO PLAGIARISM. NEED BACK TO ME ON MONDAY (28th).
Case Study on Biomedical Ethics in the Christian Narrative This assignment will incorporate a common practical tool in helping clinicians begin to ethically analyze a case. Organizing the data in this way will help you apply the four principles of principlism. Based on the “Case Study: Healing and Autonomy” and other required topic study materials, you will complete the “Applying the Four Principles: Case Study” document that includes the following: Part 1: Chart This chart will formalize principlism and the four-boxes approach by organizing the data from the case study according to the relevant principles of biomedical ethics: autonomy, beneficence, nonmaleficence, and justice. Part 2: Evaluation This part includes questions, to be answered in a total of 500 words, that describe how principalism would be applied according to the Christian worldview. Remember to support your responses with the topic study materials. APA style is not required, but solid academic writing is expected
3 DQ’S Need To Be Answers With Zero Plagiarism And 250 Word Count. Due In 4 Hours TODAY!
1. Week 3, DQ 1 How do the five stages of group development play out in your teams at your workplace? 2. Week 3, DQ 2 How might constructive disagreement (conflict) help in preventing ?groupthink? 3. Week 3, DQ 3 How important do you believe disagreement and conflict are in making an organization successful? How much disagreement and conflict do you believe an organization may tolerate before it should ?clamp down on dissent?
Medical Errors: Criminal Versus Civil Submission
Assignment Details Patient safety is a critical component of effective risk management in health care organizations. Poor patient safety practices can cost health care organizations a lot of money and increase hospital stays, costs, and loss of revenue. For this assignment, create a PowerPoint presentation on effective patient safety that includes the following: Describe the concept of patient safety and what health care organizations can do to improve it. Select 1 primary risk to effective patient safety, and provide an overview of the issue. Discuss how a health care organization can mitigate the risk associated with the issue you selected. The use of the Notes section is required with 100-150 words per section. The use of at least 4 scholarly sources is required. The use of APA style is required. Deliverable Length: 8-10 slides (not including title and reference slides)
Crisis Intervention DQ 1 Week 3
Name the DSM-IV-TR indicators necessary for a diagnosis of PTSD? (at least five of condition/symptoms, with two in each category, include specific symptoms in children) This question has different parts: You must mention the five (5) conditions, 2 characteristics in each category. Also specific symptoms in children Do you know anyone that suffers from PTSD, if you do, please share a brief description about the case. You need to support your answer with references and APA style.
Reading Summary 3
Reading Summary 3. This is not an ESSAY. No Plagiarism. Read the material and write a 250 word summary summarizing the main idea. Instructions: 3 After reading all of Chapter 8, please select ONE of the following primary source readings: ?Feminist Ethics by Alison M. Jaggar (starting on page 201) -or- ?The Ethics of Care as Moral Theory by Virginia Held (starting on page 209) -or- Write a short, objective summary of 250 words which summarizes the main ideas being put forward by the author in this selection.
Reading Summary 3
MN507 Debate Healthcar Reform Effects – PowerPoint
Debate Assignment : I am in the oposing policy MN507-1: Debate healthcare reform effects on stakeholders. Overview: In Unit 2 you chose a policy issue that interests your group. (TREATMENT OF VETERANS AFTER DEPLOYMENT AND POST TRAUMATIC STRSS DISORDER (PTSD) For this Assignment your group will be divided into two, with one side in favor of the policy change and the other side opposed to it. You will work with your debate side group member to create an audio presentation that puts forth your side’s argument (PowerPoint, Google Slides, or Prezi). Directions Your audio presentation debate should put forth a convincing argument, for or against the policy, based on your research. Your audio presentation must and address: ¢What effects will the policy change have on stakeholders? ¢Why, or why not, is this policy important? ¢What are the costs to stakeholders? ¢Important issues and research to support your position. Details: ¢Within your group, make an evaluation or judgment about the policy issue you have chosen. Then, with your co-debater, create a convincing argument, for or against the policy, based on your research. Proposition of Policy: declares that a certain policy should or should not be created. ¢Using PowerPoint, Google Slides, or Prezi, create an audio presentation of your argument (approximately 5 slides plus reference slides-APA formatted). ¢Create a transcript of your audio presentation that will be submitted to the Dropbox. ¢Your presentation should be professional in appearance and tone and reflect thoughtful and meaningful application of the policy argument. Speak clearly and convincingly. ¢You must include a minimum of (5) different scholarly works supporting your position in the debate (each side). ¢Note that this debate will be asynchronous. There will be no rebuttal. ¢Each side of the group will be graded on their work. Both students on a side will receive the same grade. ¢Submit your presentation and transcript to the Assignment Dropbox. ** This PPT assignement will be an audio presentation **
Cultural Identity Project
Conduct in-depth research on a particular cultural group. The group can be characterized by race, ethnicity, ethnicity, gender, sexual identity, socioeconomic status, disability, age, or spirituality. Must discuss development of and the current state of the cultural identity of the chosen group. APA format 10 pages
Most of us are aware that funerals are a natural event in different forms and cultural influences for all Americans, regardless of age, ethnicity, or socioeconomic status. It is important to recognize the impact of funerals on most individuals and significance specific to managing grief and loss. This discussion will examine the role of the funeral as a community support system for the bereaved person. Discuss the psychological, sociological, economic and theological philosophical aspects of the funeral process. How do each of these aspects facilitate the resolution of grief? Describe what you would include in your own obituary if you were to write it. Explain what would be your choice of final disposition of your body. Why would you choose this method, and examine the effects this choice might have on your survivors (if any)? Guided Response: Respond to at least two of your peers. Use a minimum of one scholarly source found at the Ashford Library or Google Scholar, to critically examine the procedure that you have selected and its results. Offer a substantial response to at least two other posts written by members of the class. Be sure that you cite scientific evidence to support your views. A minimum of 250 -300 words should be used for each response to other posts.
250 Words Discussion Board
Its a case study but just for 250 words. It is an essay for the psychology unit. So I need an expert in that field. 1 reference is enough but that has to be peer-reviewed.
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