Which trends did you choose, and why did you choose those?
Are those trends still pertinent today? Why or why not??
Why is it important for organizations to take a people-first approach to IT?
Idea for a vaccine to prevent Group A Strep (GAS) infections
PART 1
You have an idea for a vaccine to prevent Group A Strep (GAS) infections. You know that Streptococcus bacteria are fastidious organisms, and you don’t expect to be able to produce your protein of interest in large quantities in S. pyogenes. Based on your knowledge of GAS, design a recombinant vaccine candidate to protect against GAS infection using E. coli as your recombinant protein production organism. Your choice of GAS antigen should be something produced by GAS that is not produced by humans. Your GAS protein(s) of choice will be expressed in E. coli and then purified for use in vaccine production. (Yes, I am expecting you to find a GAS antigen on your own.) To express your protein in E. coli, you need to clone the gene(s) of interest into a plasmid under the control of the Plac promoter. Create and upload a diagram that shows each step of your design strategy and cloning process. Start with getting your gene of interest out of S. pyogenes and end with your selection and screening process in E. coli. Be sure to include all of the following:
– What GAS protein(s) will be expressed by your recombinant E. coli?
– Show all components that need to be present on your plasmid for replication, selection, screening, and for regulation by the Plac promoter.
– How do all of the parts come together?
– A selection mechanism to ensure that only recombinant E. coli expressing your plasmid can grow. Any selection mechanism is ok. Indicate what media needs to be used and what you expect to see.
– A screening mechanism to show that your gene(s) of interest is being expressed. Indicate what media needs to be used and what you expect to see.
PART 2- What S. pyogenes protein(s) are you expressing in E. coli for your recombinant vaccine candidate?
– What function does your protein provide in S. pyogenes? Why do you think it will make a good GAS vaccine candi
done
Seen
3 mins ago
I have finished designing and writing a newsletter for the Finish School in Qatar. I need some professional writer
I have finished designing and writing a newsletter for the Finish School in Qatar.
I need some professional writer to read it and see if it need any development. Then after that, we can discuss the rate for that. Sure I will not pay for the pages because I have already written them by myself. Just I need somebody who is professional to look at it and suggest if any changes could be added.
I need any comment or suggested changes to be done in in a three hours period only.
The essay is open-ended and can be from an analytic or relational view. Listen to the Oral Arguments and Essay
Writing Assignment Help The essay is open-ended and can be from an analytic or relational view. Listen to the Oral Arguments and write a 350-word minimum essay over what part of the argument peaked your interest. No citations necessary. The audio for the oral arguments is located on https://www.oyez.org/cases/2014/14-556 . Thank you!
Idea for a vaccine to prevent Group A Strep (GAS) infections
PART 1
You have an idea for a vaccine to prevent Group A Strep (GAS) infections. You know that Streptococcus bacteria are fastidious organisms, and you don’t expect to be able to produce your protein of interest in large quantities in S. pyogenes. Based on your knowledge of GAS, design a recombinant vaccine candidate to protect against GAS infection using E. coli as your recombinant protein production organism. Your choice of GAS antigen should be something produced by GAS that is not produced by humans. Your GAS protein(s) of choice will be expressed in E. coli and then purified for use in vaccine production. (Yes, I am expecting you to find a GAS antigen on your own.) To express your protein in E. coli, you need to clone the gene(s) of interest into a plasmid under the control of the Plac promoter. Create and upload a diagram that shows each step of your design strategy and cloning process. Start with getting your gene of interest out of S. pyogenes and end with your selection and screening process in E. coli. Be sure to include all of the following:
– What GAS protein(s) will be expressed by your recombinant E. coli?
– Show all components that need to be present on your plasmid for replication, selection, screening, and for regulation by the Plac promoter.
– How do all of the parts come together?
– A selection mechanism to ensure that only recombinant E. coli expressing your plasmid can grow. Any selection mechanism is ok. Indicate what media needs to be used and what you expect to see.
– A screening mechanism to show that your gene(s) of interest is being expressed. Indicate what media needs to be used and what you expect to see.
PART 2- What S. pyogenes protein(s) are you expressing in E. coli for your recombinant vaccine candidate?
– What function does your protein provide in S. pyogenes? Why do you think it will make a good GAS vaccine candid
done
Seen
3 mins ago
https://www.youtube.com/watch?v=TTJA3LLl-6I
https://www.youtube.com/watch?v=TTJA3LLl-6I